mouse recombinant leukemia inhibitory factor (R&D Systems)

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R&D Systems mouse recombinant leukemia inhibitory factor
Mouse Recombinant Leukemia Inhibitory Factor, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/leukemia+inhibitory+factor+lif/Recombinant+Mouse+LIF+Protein%2C+CF/pmc12999487-375-17-22
Average 94 stars, based on 3 article reviews

mouse recombinant leukemia inhibitory factor - by Bioz Stars, 2026-09

94/100 stars

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Control:

Article Title: Human mesenchymal stem cells promote human osteoclast differentiation from CD34+ bone marrow hematopoietic progenitors.
Article Snippet: .. To assess the effects of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] and cytokines on Ocl formation, 1,25(OH)2D3 (BIOMOL Research Laboratories, Inc., Plymouth Meeting, PA); or antibodies to human interleukin (IL)-6, IL-11, leukemia inhibitory factor (LIF), or M-CSF; or control IgG (R&D Systems, Minneapolis, MN) were added to the coculture and maintained through the coculture period for up to 3 weeks. .. To determine the role of cell contact in Ocl formation, experiments were performed in which HSCs and hMSCs were cultured in either the same chamber or in chambers separated by 0.45-mm porous membrane (Becton Dickinson and Co., Bedford, MA) for 3 weeks.

Incubation:

Article Title: Dihydroartemisinin attenuates osteoarthritis by inhibiting abnormal bone remodeling and angiogenesis in subchondral bone.
Article Snippet: .. Sagittal sections that had been paraffin‐embedded were incubated overnight at 4 ̊C with primary antibodies against matrix metalloproteinase‐13 (MMP‐13) (1:100; product code ab39012; Abcam), vascular endothelial growth factor A (VEGFA) (1:100; product code ab52917; Abcam), leukemia inhibitory factor (LIF) (1:100; cat. no. AB‐449‐NA; R&d Systems, Inc.), sclerostin (1:50; product code ab63097; Abcam), β‐catenin (1:100; cat. no. 51067‐2‐AP; ProteinTech Group, Inc.), RANKL (1:100; product code ab216484; Abcam) and cd31 (1:25; product code ab28364; Abcam). .. For immunohistochemical staining, sections were processed using a two‐step IHc detection reagent (ZSGB‐Bio; OriGene Technologies, Inc.).

Article Title: Dihydroartemisinin attenuates osteoarthritis by inhibiting abnormal bone remodeling and angiogenesis in subchondral bone
Article Snippet: .. Sagittal sections that had been paraffin-embedded were incubated overnight at 4°C with primary antibodies against matrix metalloproteinase-13 (MMP-13) (1:100; product code ab39012; Abcam), vascular endothelial growth factor A (VEGFA) (1:100; product code ab52917; Abcam), leukemia inhibitory factor (LIF) (1:100; cat. no. AB-449-NA; R&D Systems, Inc.), sclerostin (1:50; product code ab63097; Abcam), β-catenin (1:100; cat. no. 51067-2-AP; ProteinTech Group, Inc.), RANKL (1:100; product code ab216484; Abcam) and CD31 (1:25; product code ab28364; Abcam). .. For immunohistochemical staining, sections were processed using a two-step IHC detection reagent (ZSGB-Bio; OriGene Technologies, Inc.).

Clinical Proteomics:

Article Title: The Role of Pretreatment Serum Interleukin 6 in Predicting Short-Term Mortality in Patients with Advanced Pancreatic Cancer
Article Snippet: .. The quantification of cytokines in the plasma utilized ELISA kits designed for IL-6 (#DY206-05), leukemia inhibitory factor (LIF) (#DYF00B), IL-1β (#DY201-05), IL-10 (#DY217B-05), granzyme B (#DY2906-05), and interferon-gamma (#DY285B-05), all sourced from R&D Systems, Minneapolis, MN, USA, and were employed following the manufacturer’s specified protocols. .. Additionally, levels of soluble IL-6 receptor α (sIL-6Rα) protein were measured employing DuoSet ELISA kits for each respective protein (#DY227, R&D Systems, Minneapolis, MN, USA), adhering to the provided instructions.

Enzyme-linked Immunosorbent Assay:

Article Title: Variations of clonal marrow stem cell lines established from human bone marrow in surface epitopes, differentiation potential, gene expression, and cytokine secretion.
Article Snippet: Bone marrow has been considered to contain many different types of progenitor or stem cells.. This study aims to establish a new strategy that provides for the rapid establishment of human clonal marrow stem cell (hcMSC) lines with a relatively small amount of bone marrow aspirate and to characterize newly generated hcMSC lines for their cell phenotype, differentiation potential, lineage-specific gene expression, and cytokine secretion.. Human cMSC lines were generated with human bone marrow aspirates using a new protocol, called the subfractionation culturing method.

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